Analysis of the canine EDAR gene and exclusion as a candidate for the hairless phenotype in the Chinese Crested dog.

Source/description of primers: Chromogranins mediate sorting and aggregation of hormones and neuropeptides into secretory granules in the regulated secretory pathway. Chromogranin B (CHGB)/secretogranin I is found in secretory granules/large dense-cored vesicles throughout cells of the endocrine and nervous system. Because of its role in hormone secretion, CHGB is critically involved in reproductive function. Two cDNA clones, one containing the full coding region (pCHGB1; GenBank accession no. AY613916) and another containing a partial coding region (pCHGB2; GenBank accession no. AY623647) of the porcine CHGB, were isolated from the Meat Animal Research Center (MARC) 2PIG expressed sequence tag library. A silent single nucleotide polymorphism (SNP) in the coding region (nucleotide 1568, AY613916; and nucleotide 876, AY623647) was identified by comparing the sequences of the two clones. Primers were designed to amplify a 941 bp product in the coding region of the gene. The forward (F) and reverse (R) primers correspond to bases 1056–1075 and 1996– 1975 of the porcine CHGB cDNA (GenBank accession no. AY613916).